Figure 2.

Gastrointestinal Gpr64-mCherry positive cells are enteric nerves and tuft cells Representative fluorescence microscopy images of Gpr64^mCherry tissue sections immunostained for selected markers specific for tuft cells, microvillous cells, and enteric nerves. A) GI tract Gpr64^mCherry-positive cells (red) stained with doublecortin like kinase 1 (DCLK1), acetylated alpha Tubulin (AC-TUB), cytokeratin 18 (CK18), hematopoietic prostaglandin D synthase (HPGDS), prostaglandin D2 Synthase 1 (PTGS1) and prostaglandin D2 Synthase 2 (PTGS2) antibodies (green) and counterstained with DAPI (blue). Bar = 5 μm. B) Gpr64^mCherry-positive submucosal and myenteric nerves immunostained with anti-PGP9.5 Bar = 50 μm. C) Respiratory tract Gpr64^mCherry cells stained with DCLK1 and CK18 antibodies (scale bar, 5 μm). Fourth panel displays olfactory epithelium isolated from the double transgenic Trpm5^GFP;Gpr64^mCherry reporter mouse with both Gpr64 promoter-driven mCherry and Trpm5 promoter-driven GFP expression. Bar = 20 μm. Male mice n = 3. Ab, Antibody. D) Small intestinal sections from GPR64^DTA control mice (upper row) and Vil-CreERT2;GPR64^DTA mice (lower row) before and 48 h after tamoxifen induced cell ablation. Tuft cells visualized with anti-DCLK1 (red). Bar = 100 μm. Per group n = 6–8 mice.