Figure 6.

Comparative transcriptional analysis of young vs. mature intestinal tuft cells Heatmap depicting the log2 (x+1) normalized expression of 50 genes with the highest log2 fold A) downregulation or B) upregulation between young tuft cells (Trpm5^GFP-positive, Gpr64^mCherry-negative) and mature tuft cells (Trpm5^GFP-positive, Gpr64^mCherry-positive) from Trpm5^GFP;Gpr64m^Cherry proximal small intestine (all plotted genes had statistically significant log2 fold changes in mature versus young cells, P < 0.01). C) Volcano plot. The vertical dimension represents the log10-transformed p-values (with inverted sign) from a two-sided Mann–Whitney–Wilcoxon geneset enrichment test (Materials and Methods). The horizontal dimension represents the weighted mean of the log2 fold change in expression of the geneset genes in old versus young tuft cells, where each gene's contribution is weighted by the log-transformed p-value (with the sign reversed) corresponding to the log2 fold change. Thus green bubbles left of zero represent genesets which are downregulated in old versus young tuft cells, and red bubbles represent upregulated genesets. The size of the bubble represents the number of genes in the geneset. Mice n = 5.