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. 2021 May 7;11:9803. doi: 10.1038/s41598-021-89379-x

Figure 2.

Figure 2

γS-deficient cells cannot process ACE2ΔE. (A) Western blots from Presenilin 1/2 double KO (Psen1/2 dKO) or Nicastrin KO (Ncstn KO) MEFs and their wild type (WT) controls. Cells were transfected with ACE2-C9 with or without TMPRSS2. ACE2ΔE generated in the absence of TMPRSS2 has a higher molecular weight, which corresponds to ADAM17-mediated shedding. (B) Representative immunofluorescence images of WT MEFs transfected with ACE2-C9. (C) Representative immunofluorescence images of WT, Psen1/2 dKO and Ncstrn KO MEFs transfected with ACE2-C9 and TMPRSS2. (D) GFP fluorescence in WT or Ncstrn KO MEFs co-expressing ACE2-GFP and TMPRSS2. (E) Representative immunofluorescence images of WT MEFs transfected with ACE2-C9 and TMPRSS2 in the presence of GSI. Scale bars: 10 µm.