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. 2021 May 7;11:9766. doi: 10.1038/s41598-021-89137-z

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Showing results of targeted metabolomics analysis of cell culture supernatant of N9 cells treated with ATP100μM in positive and negative mode. Metabolomic analysis of ATP (100 μM) treated N9 cells analyzed in the positive mode showing. (a) Showing heat map of significant differential metabolites between control sets and ATP (100 µM) treated sets in positive mode. (b) Unbiased PCA clustering of control (red color) and ATP (100 µM) treated (green color) sets in positive mode as represented by a score plot. (c) Random Forest analysis. (d) List of pathways to which differential metabolites belong to are obtained at an FDR of 0.25 in both positive and negative mode and common to both positive and negative. (e) Showing heat map of significant differential metabolites between control sets and ATP (100 µM) treated sets in negative mode. (f) Unbiased PCA clustering of control (red color) and ATP (100 µM) treated (green color) sets in negative mode as represented by a score plot. (g) Random Forest analysis. (h) Overlapping metabolic pathways between POAG and N9 cell culture supernatant. (a–c) and (e–g) was generated using MetaboAnalyst 5.0 (Version-5.0; URL link: https://www.metaboanalyst.ca/). (h) was created using VENNY ^2.1(Version-2.1; URL link- https://bioinfogp.cnb.csic.es/tools/venny/).