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. 2021 May 7;7:95. doi: 10.1038/s41420-021-00479-z

Fig. 4. Alterations in TRIP12 level results in loss of cellular polarity, increase in single-cell dislodgment, increase in cell motility, and sensitivity to anoikis.

Fig. 4

a Relative expression of the mRNA levels of EMT markers in MCF10A cells stably depleted of TRIP12 and with LHCX-vector expression or LHCX-TRIP12 expression. Relative expression levels were normalized to Actin mRNA levels and data quantified relative to shControl LHCX vector. (N = 3). Data represent means ± SEM. * p value < 0.05; ** p value < 0.01. b Representative cell tracking snapshot images of cell migration/ wound-healing assay for MCF10A shControl and shTRIP12-2(6427–6447), with either LHCX-vector or LHCX-TRIP12 expression, at midpoint to wound closure. Fifteen single cell tracks are represented with different colors in each image. Images were taken at 10X magnification. The scale bar at bottom right corner = 100 µm. c Quantification of the number of single cells in the remaining wound area from images when the remaining wound area is 35–55% of the total area. (N = 6). Data represent means ± SEM. ** p value < 0.01; *** p value < 0.001. d Velocity of tracked MCF10A shControl and shTRIP12-2(6427–6447), with either LHCX-vector or LHCX-TRIP12 expression. Fifteen cell tracks per condition were used for acquiring velocity data. Data represent means ± SEM. ** p value < 0.01; *** p value < 0.001.