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. 2021 May 7;7:95. doi: 10.1038/s41420-021-00479-z

Fig. 5. TRIP12 inhibits ZEB1/2 gene expression to regulate EMT and cellular behavior.

Fig. 5

a Relative expression of the mRNA levels of EMT transcription factors in MCF10A cells stably depleted of TRIP12. Relative expression levels were normalized to Actin mRNA levels and data quantified relative to shControl. (N = 7). Data represent means ± SEM. ** p value < 0.01; *** p value < 0.001. b Relative expression of the mRNA levels of EMT markers in MCF10A cells stably depleted of TRIP12 and with ZEB1/2 depletion. Relative expression levels were normalized to Actin mRNA levels and data quantified relative to shControl siControl. (N = 3). Data represent means ± SEM. * p value < 0.05; ** p value < 0.01; *** p value < 0.001. c Representative cell tracking snapshot images of cell migration/ wound-healing assay for MCF10A shTRIP12-2(6427–6447), with either siControl or siZEB1/2, at midpoint to wound closure. Fifteen single cell tracks are represented with different colors in each image. Images were taken at 10X magnification. The scale bar at the bottom right corner = 100 µm. d Quantification of the number of single cells in the remaining wound area from images when the remaining wound area is 35–45% of the total area. (N = 5). Data represent means ± SEM. ***, p value < 0.001. e Velocity of tracked MCF10A shTRIP12-2(6427–6447) siControl and siZEB1/2 cells. Fifteen cell tracks per condition were used for acquiring velocity data. Data represent means ± SEM. *** p value < 0.001.