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. 2021 May 7;11:9775. doi: 10.1038/s41598-021-89163-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal image of stained nuclei (DAPI staining; blue) and cell bodies (NeuroTrace green fluorescent Nissl staining, green) of (a1) control (non-irradiated) and (b1) infrared-irradiated mouse brain hemisphere. Control area (a2) corresponding to the (b2) localized IR-irradiated area, used for the detection of cell bodies. Identified cell bodies in (a3) control and (b3) irradiated area. (c) Box plot of cell density values per 0.1 mm² for control and IR stimulation hemispheres. (d) Cellular density values (number of cells per 0.1 mm²; mean ± SD) for cortical layers in control and IR stimulation hemispheres, showing no significant difference (t-test, N = 3 mice). Scale bar: 100 µm (figure created from Excel 2016, Powerpoint 2016 and ImageJ).