Fig. 5.

Cell respiration and partial metabolic adaptation to osmotic stress. Oxygen consumption was measured with increasing concentrations of KCl (A) and NaCl (B) in the medium. Cells were exposed to the indicated KCl (2 M) or NaCl (2 M) concentrations, and oxygen consumption was measured immediately after osmotic stress or 10 min after (subscript i for incubated). Cells (100 mg wet weight) were added at the beginning of the experiment to a medium containing 3 mM MES-TEA buffer, pH 6.0, final volume 5 mL. Twenty millimoles glucose was added at the beginning of each tracing. Dissolved oxygen saturation was 400 natg/mL in a final volume of 5 mL, amounting to a total of 2000 natg in the incubation chamber. Oxygen consumption was normalized to the consumption of oxygen in the absence of KCl or NaCl. Data show the means of four independent observations. ^*Asterisks show statistical differences among data points, compared to the values of the control without salt in a t-test