Fig. 1.

Functional verification of purified rPLO. a rPLO at different dilution was incubated with or without 8 μg/μl cholesterol for 1 h, and a 2% sheep red blood cell suspension was challenged with the abovementioned mixture for 1 h. One AU indicates a final concentration of 2 ng/μl rPLO in this assay. b MBCD at different dilution was incubated with a 2% sheep red blood cell suspension for 12 h, after which rPLO was added and incubated with the mixture for 1 h. The optical density at a wavelength of 405 nm was measured using a microplate reader. Error bars represent SEM of three independent experiments, *P < 0.05