Fig. 5.

Autophagy and NLRP3 participated in regulating of rPLO induced inflammatory response genes expression. gESCs were challenged with control medium and medium containing 0.1 μg/ml rPLO, 0.5 μg/ml LPS, or costimulation for 3 h. a The cell samples were analyzed by Western blotting with anti-LC3, anti-NLRP3 and anti-β-actin (loading control) antibodies. The protein levels of target proteins relative to the protein level of β-actin were determined by densitometry. b–d mRNA levels of targets normalized to the levels of GAPDH were determined by RT-qPCR. P < 0.05. e gESCs were preincubated with CQ at 20 μM for 3 h, after which using rPLO and LPS costimulation for another 3 h, and relative mRNA levels were determined by RT-qPCR. Error bars represent the SEM of three independent experiments, *P < 0.05 and **P < 0.01