Figure 1.

mADSC can produce IL-33 with IL-1β stimulation

(A) Il33 mRNA expression in mADSC stimulated for 6 h with different cytokines as indicated. (2 ng/mL IL-1β, 10 ng/mL IL-4, 10 ng/mL IL-5, 50 ng/mL IL-6, 10 ng/mL IL-7, 10 ng/mL IL-12, 10 ng/mL IL-13, 10 ng/mL IL-15, 10 ng/mL IL-21, 10 ng/mL IL-22, 10 ng/mL IL-23, 10 ng/mL IL-25,10 ng/mL IFNγ, 2 ng/mL TGF-β, 10 ng/mL TNFα, 10 ng/mL LPS).

(B) Il33 mRNA can be produced in mADSC stimulated for 6 h with 10 ng/mL IL-1β.

(C) Il33 mRNA in mADSC stimulated for 6 h with different dose of concentration of IL-1β (0, 0.002, 0.02, 0.2, 2 and 20 ng/mL).

(D) Western blot of IL-33 and β-actin in mADSC stimulated with or without 2 ng/mL IL-1β for 24 or 48 h

(E) ELISA of IL-33 in mADSC lysate and supernatant stimulated with or without 10 ng/mL IL-1β for 72 h. Data are pooled from three (A and C) independent experiments or are representative of three (B, D, and E) independent experiments. In (A), (B), (C), and (E) one-way ANOVA was used. Bars, mean; error bars, SEM; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.