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. 2021 Apr 16;24(5):102446. doi: 10.1016/j.isci.2021.102446

Figure 3.

Figure 3

IL-33 from mADSC affects ST2 expression on Treg in vitro

(A) Il33 mRNA expression in ADSC from WT and Il33−/− mice stimulated for 6 h with or without 10 ng/mL IL-1β.

(B) ELISA of IL-33 protein in mADSC stimulated with 10 ng/mL IL-1β for 72 h

(C) Il1rl1 mRNA expression in CD4+CD25+ T cell from spleen with anti-CD3/CD28 plus stimulated with or without ADSC lysate from WT or Il33−/− mice or ADSC soap from WT mice with anti-IL-33 for 24 h.

(D) Frequency and absolute number of Foxp3+ T cells in CD4+CD25+ T cell from spleen with anti-CD3/CD28 plus stimulated with or without ADSC lysate from WT or Il33−/− mice or ADSC soap from WT mice with anti-IL-33 for 72 h.

(E) Frequency and absolute number of ST2+ T cells in CD4+Foxp3+ T cell from spleen with anti-CD3/CD28 plus stimulated with or without ADSC lysate from WT or Il33−/− mice or ADSC soap from WT mice with anti-IL33 for 72 h. Data are pooled from three (A, C, D, and E) independent experiments or are representative of three (B) independent experiments. In (B), Student's t test was used. In (A), (C), (D), and (E), one-way ANOVA was used. Bars, mean; error bars, SEM; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.