Table 1.

Effect of in ovo inoculation of a Marek's vaccine and three individual B. subtilis serotypes at ~ 10⁶ cfu/ 50µL on hatch parameters¹.

Hatch parameters	MV (T1)	B. subtilis ATCC 6051 (T2)	B. subtilis ATCC 8473 (T3)	B. subtilis ATCC 9466 (T4)	P-value	SEM
Hatch of transfer	94.7a	17.3b	96.1a	96.2a	< 0.0001	1.215
Early dead embryos	0.45	0	0	0	0.0652	0.118
Mid dead embryos	0.58	0	0	0.21	0.1789	0.188
Late dead embryos	3.10b	12.4a	2.40b	2.30b	0.0023	1.401
Pipped embryos	1.15b	69.5a	1.32b	0.85b	< 0.0001	0.551
Contaminated embryos	0	0.82	0.22	0.42	0.0628	0.182
Avg. Egg Weight (g)2	63.63	63.97	64.23	64.04	0.4540	0.351
Avg. Chick Weight (g)3	44.1a	42.2b	43.6a	43.2a	0.0048	0.374

^a-bMeans in a row not sharing a common superscript are different (P ≤ 0.005).

¹N=3, observed means were calculated using each GQF hatcher unit as a replicate (∼180 eggs/GQF; ∼540 eggs/treatment).

²Avg. egg weight on d 18 prior to the in ovo inoculation

³Avg. chick weight on the day of hatch