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Transfer buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris | 96 mM | 7.2 g |
| Glycine | 12 mM | 1.46 g |
| Methanol | 20% | 200 mL |
| ddH2O | n/a | Add up to 1 L |
| Total | n/a | 1 L |
Dissolve and store at 4°C for 6 months. 20% methanol waste must be disposed following proper procedure.
