RIPA/SDS

Reagent	Final concentration	Amount
RIPA buffer	n/a	9.1 mL
cOmplete Protease Inhibitor Cocktail (25X solution)	1X	0.4 mL
20% Sodium dodecyl sulfate	1%	0.5 mL
Total	n/a	10 mL

Freshly prepare before use.