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. 2021 Apr 29;2(2):100501. doi: 10.1016/j.xpro.2021.100501

2X Sample buffer

Reagent Final concentration Amount
Novex™ Tricine SDS Sample Buffer (2X) n/a 910 μL
cOmplete Protease Inhibitor Cocktail (25X solution) 1X 40 μL
2-mercaptoethanol 5% 50 μL
Total n/a 1 mL

Freshly prepare before use.