REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-β-amyloid, 1–16 antibody, clone 6E10 (mouse ascites)	BioLegend (Signet, Covance)	Cat# SIG-39300-1000; RRID: AB_662809
Amyloid β (N) (82E1) Anti-Human Mouse IgG MoAb antibody	Immuno-Biological Laboratories	Cat# 10323; RRID: AB_10707424
Sheep Anti-Mouse IgG, Whole Ab ECL Antibody, HRP Conjugated	GE Healthcare	Cat# NA931; RRID: AB_772210
Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preabsorbed Antibody	Abcam	Cat# ab150117; RRID: AB_2688012
Chemicals, peptides, and recombinant proteins
Agar (for fly food)	Ina Food Industry Co., Ltd.	Cat# S-6
Sucrose (for fly food)	Nissin Sugar Co., Ltd.	N/A
Potassium tartrate	Sigma-Aldrich	Cat# 24-5650-5
Calcium chloride	Sigma-Aldrich	Cat# 05-0580-5
Glucose	Nacalai Tesque	Cat# 16805-64
Corn meal (for fly food)	Oriental Yeast Co., Ltd.	Cat# 02801500
Yeast (for fly food)	Mitsubishi Tanabe Pharma	Product name: Ebios
Methyl 4-hydroxybenzoate	FUJIFILM Wako Pure Chemical Corporation	Cat# 132-02635
Ethanol	FUJIFILM Wako Pure Chemical Corporation	Cat# 057-00451
Phosphoric acid	Nacalai Tesque	Cat# 27618-55
Propionic acid	FUJIFILM Wako Pure Chemical Corporation	Cat# 163-04726
16% Paraformaldehyde	Electron Microscopy Sciences	Cat# 15710
Sodium bicarbonate	Sigma-Aldrich	Cat# 28-1850
FSB (1-fluoro-2,5-bis(3-carboxy-4-hydroxystyryl)benzene, 1% DMSO solution)	Dojindo	Cat# F308
Glycerol	FUJIFILM Wako Pure Chemical Corporation	Cat# 075-00616
Lithium carbonate	Nacalai Tesque	Cat# 20619-42
Formic acid	Kanto Chemical Co., Inc.	Cat# 16064-00
Sodium chloride (NaCl)	Kanto Chemical Co., Inc.	Cat# 37144-86
Potassium chloride (KCl)	Sigma-Aldrich	Cat# 24-4290
Disodium hydrogen phosphate (Na2HPO4)	FUJIFILM Wako Pure Chemical Corporation	Cat# 193-02845
Potassium dihydrogen phosphate (KH2PO4)	FUJIFILM Wako Pure Chemical Corporation	Cat# 169-04245
Tris (hydroxymethyl) aminomethane (Tris)	Nacalai Tesque	Cat# 35434-21
Hydrochloric acid (HCl)	FUJIFILM Wako Pure Chemical Corporation	Cat# 080-01066
Sodium deoxycholate	FUJIFILM Wako Pure Chemical Corporation	Cat# 194-08311
Polyoxyethylene (10) octylphenyl ether (Triton X-100)	FUJIFILM Wako Pure Chemical Corporation	Cat# 168-11805
Tricine	Santa Cruz Biotechnology	Cat# SC-216103A
Sodium dodecyl sulfate	Nacalai Tesque	Cat# 31606-75
Glycine	Nacalai Tesque	Cat# 17109-35
Methanol	Kanto Chemical Co., Inc.	Cat# 25183-70
Tween-20	Kanto Chemical Co., Inc.	Cat# 40350-02
Skim milk	Morinaga Milk	N/A
cOmplete Protease Inhibitor Cocktail	Merck	Cat# 11697498001
2-Mercaptoethanol	Nacalai Tesque	Cat# 21417-52
Paraffine wax	Sakura Finetek Japan	Cat# 7810
Multi mount 220	Matsunami Glass Ind., Ltd	Cat# FM22001
SlowFade™ Gold Antifade Mount	Thermo Fisher Scientific	Cat# S36936
Normal goat serum	Jackson ImmunoResearch Laboratories	Cat# 005-000-121
Xylene	Nacalai Tesque	Cat# 36612-93
Critical commercial assays
ECL Prime Western Blotting Detection Reagents	GE Healthcare	Cat# RPN2236
Novex™ Tricine SDS Sample Buffer (2X)	Thermo Fisher Scientific	Cat# LC1676
Novex™ 10 to 20%, Tricine, 1.0 mm, Mini Protein Gel, 10-well	Thermo Fisher Scientific	Cat# EC6625BOX
Hematoxylin Solution, Mayer’s	Sigma-Aldrich	Cat# MHS16
Eosin Y solution	Sigma-Aldrich	Cat# HT110132
Experimental models: organisms/strains
D. melanogaster: UAS-Aβ42	(Iijima et al., 2004; Iijima and Chiang, 2008)	N/A
D. melanogaster: elav-GAL4	Bloomington Drosophila Stock Center	BDSC: 458; FlyBase: FBst0000458
D. melanogaster: w1118	Vienna Drosophila Resource Center	VDRC: 60000
Software and algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Prism 9	GraphPad	https://www.GraphPad.com
Other
Auto tissue processor	Sakura Finetek Japan	Model: ETP-150C
Tissue Embedding Console System	Sakura Finetek Japan	Model: Tissue-Tek TEC
Rotary microtome	Yamato Kohki Industrial Co., Ltd.	Model: RX-860
Microtome blade	Feather	Cat# S35
Forceps	DUMONT	Cat# 0108-5-PO
Vacuum centrifuge concentrator	Eppendorf	Model: Vacufuge