Skip to main content
. 2021 Apr 29;2(2):100501. doi: 10.1016/j.xpro.2021.100501

RIPA buffer

Reagent Final concentration Amount
1M Tris-HCl buffer (pH 8.0) 50 mM 10 mL
Sodium deoxycholate 0.5% 1 g
Triton X-100 1% 2 mL
NaCl 150 mM 1.75 g
ddH2O n/a Add up to 200 mL
Total n/a 200 mL

Dissolve and store at 4°C for a month.