Skip to main content
. 2021 Feb 18;120(8):1454–1468. doi: 10.1016/j.bpj.2021.01.038

Figure 2.

Figure 2

Activity of isolated pacemaker near critical temperatures. (A–C) Intracellular recordings of the PD neuron in the presence of PTX across a range of temperatures. Scale, 10 mV with dash at −50 mV. (D–F) Burst frequency (D), duty cycle (E), and minimal voltage during oscillation (F) of PD neuron from 13 preparations are plotted as a function of temperature. Duty cycle is computed from intracellular traces as burst duration normalized to period of oscillation. Duty cycle becomes undefined for single spike bursts. Colored lines correspond to example experiments with the same colors in (A)–(C). (G–H) Above: average burst frequency (G) and duty cycle (H) across preparations are plotted as a function of distance, in degrees Celsius, to transition to silence. Error bars represent SDs. Not all cells were recorded for full 3° before transition (see Materials and methods). Below: coefficients of variation for burst frequency and duty cycle, respectively, calculated from above plots and aligned to transition to silence. ∗p < 0.05; ns, not significant. (G, top) Frequency is predicted by proximity to transition using linear regression; (bottom) p = 0.0342, two-sample F-test for equal variance. (H, top) Duty cycle is predicted by proximity to transition using linear regression; (bottom) p = 0.0069, two-sample F-test for equal variance. To see this figure in color, go online.