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. 2021 Feb 18;120(8):1454–1468. doi: 10.1016/j.bpj.2021.01.038

Figure 3.

Figure 3

Activity of isolated pacemaker in acidic pH. (A–C) Intracellular recordings of the PD neuron in the presence of PTX across range of acidic pH. Scale, 10 mV with dash at −50 mV. (D–F) Burst frequency (D), duty cycle (E), and minimal voltage during oscillation (F) of PD neuron are plotted as a function of pH from 15 preparations. Duty cycle is computed as in Fig. 2. Colored traces correspond to example experiments of the same color in (A)–(C). Frequency and duty cycle are only plotted when cell is bursting. (G) Above: average burst frequency (G) and duty cycle (H) across preparations plotted as a function of distance to transition to tonic spiking. Error bars represent SDs. Not all cells were recorded for a range of 1 pH. Below: coefficients of variation for burst frequency and duty cycle, respectively, calculated from the above plot and aligned to transition to tonic spiking. ∗p < 0.05; ns, not significant. (G, top) Frequency is predicted by proximity to transition using linear regression; (bottom) p = 0.3757, two-sample F-test for equal variance. (H, top) Duty cycle is predicted by proximity to transition using linear regression; (bottom) p = 0.2893, two-sample F-test for equal variance. To see this figure in color, go online.