Table 1 ∣.
Technique | Tag | Cell culture | In vivo | Cell-type specificity |
References |
---|---|---|---|---|---|
Radiolabeled amino acid labeling | Radiolabeled amino acids (e.g., 35S-methionine) | Yes. Methionine-free conditions | No | No | Esposito & Kinzy32, Schick33 |
FUNCAT | Non-canonical amino acids (e.g., HPG, AHA) | Yes. Methionine-free conditions | Yes. Specialized chow free of methionine and containing non-canonical amino acids or very high doses of amino acid analogs | Yes | Dieterich et al.36 |
BONCAT | Non-canonical amino acids | Yes. Methionine-free conditions | Yes. Specialized chow free of methionine and containing non-canonical amino acids | No | Dieterich et al.37,38 |
QuaNCAT | Non-canonical amino acids | Yes. Methionine-free conditions | Yes. Specialized chow free of methionine and containing non-canonical amino acids | No | Howden et al.39 |
OP-Puro | O-propargyl-puromycin | Yes | Yes | Yes | Liu et al.6, Signer et al.7 |
SUnSET | Puromycin | Yes | Yes, but it can be applied to only some cell types | Yes | Goodman & Hornberger42, Schmidt et al.43, Goodman et al.44 |
PUNCH-P | Biotinylated-puromycin | Yes | No. Tissues can be labeled ex vivo | No | Aviner et al.45 |
SILAC | Isotopes | Yes | Yes. Administration of radiolabeled amino acids | No | Ong et al.46, Ong & Mann47, Kruger et al.48 |