Table 1 ∣.

Comparison of different methods to measure protein synthesis

Technique	Tag	Cell culture	In vivo	Cell-type specificity	References
Radiolabeled amino acid labeling	Radiolabeled amino acids (e.g., 35S-methionine)	Yes. Methionine-free conditions	No	No	Esposito & Kinzy32, Schick33
FUNCAT	Non-canonical amino acids (e.g., HPG, AHA)	Yes. Methionine-free conditions	Yes. Specialized chow free of methionine and containing non-canonical amino acids or very high doses of amino acid analogs	Yes	Dieterich et al.36
BONCAT	Non-canonical amino acids	Yes. Methionine-free conditions	Yes. Specialized chow free of methionine and containing non-canonical amino acids	No	Dieterich et al.37,38
QuaNCAT	Non-canonical amino acids	Yes. Methionine-free conditions	Yes. Specialized chow free of methionine and containing non-canonical amino acids	No	Howden et al.39
OP-Puro	O-propargyl-puromycin	Yes	Yes	Yes	Liu et al.6, Signer et al.7
SUnSET	Puromycin	Yes	Yes, but it can be applied to only some cell types	Yes	Goodman & Hornberger42, Schmidt et al.43, Goodman et al.44
PUNCH-P	Biotinylated-puromycin	Yes	No. Tissues can be labeled ex vivo	No	Aviner et al.45
SILAC	Isotopes	Yes	Yes. Administration of radiolabeled amino acids	No	Ong et al.46, Ong & Mann47, Kruger et al.48