. Author manuscript; available in PMC: 2021 May 8.

Published in final edited form as: Nat Protoc. 2019 Feb;14(2):441–460. doi: 10.1038/s41596-018-0100-z

Table 2 ∣.

Antibody staining for HSCs and CMP/GMP/MEP immunophenotyping

HSC: Antibody	Volume/million cells (μl)	CMP/GMP/MEP: Antibody	Volume/million cells (μl)
CD3-FITC^a	0.2	CD3-FITC^a	0.2
CD8-FITC^a	0.2	CD4-FITC^a	0.2
CD5-FITC^a	0.2	CD8-FITC^a	0.2
Gr1-FITC^a	0.2	CD11b(Mac1)-FITC^a	0.2
Ter119-FITC^a	0.2	Gr1-FITC^a	0.2
CD45R(B220)-FITC^a	0.4	Ter119-FITC^a	0.2
CD48-FITC	0.2	CD45R(B220)-FITC^a	0.4
CD117-e780	1	CD127-FITC	0.4
Sca-1-PerCp-Cy5.5	1	Sca1-APC	1
CD150-APC	1	CD34-AF700	1
		CD117-e780	1
		CD16/32-PerCp-Cy5.5	0.4
Recommended single-color control antibodies
Gr1-FITC
CD11b-e780
CD45R(B220)-PerCp-Cy5.5
CD11b(Mac1)-APC
CD34-AF700

Indicates lineage-specific antibodies.