Skip to main content
. 2020 Nov 20;11(4):1047–1055. doi: 10.1016/j.apsb.2020.11.008

Figure 2.

Figure 2

Comparison of cellular uptake and integrity of two different-sized PLGA nanoparticles. (A) Counts of DCs that absorbed DiI-loaded PLGA nanoparticles (DiI: 5 μg/mL) after co-incubated with S-NPs (left) and L-NPs (right) for 1, 2 and 4 h. (B) Mean fluorescence intensity of DCs incubated with DiI-loaded PLGA nanoparticles (DiI: 5 μg/mL) for 1, 2 and 4 h, respectively. Data are presented as mean ± SD (n = 3), ∗∗∗∗P < 0.0001, ∗∗P < 0.01. (C) Confocal laser scanning imaging of DCs, which were respectively pre-incubated with DiO/DiI-loaded PLGA nanoparticles (DiO/DiI: 10 μg/mL) for 1 h as the starting point, and then medium was removed to observe the FRET up to 4 h. (Scale bar = 100 μm). (D) FRET ratio changes with time for S-NPs and L-NPs. Data are presented as mean ± SD (n = 3).