Figure 4.

In vivo integrity of DiO/DiI-loaded PLGA nanoparticles in skins and SDLN after IMQ-induced psoriasis-like mice were intradermally injected of different-sized DiO/DiI-loaded PLGA nanoparticles at 8 h. Confocal laser scanning imaging of IMQ-induced psoriasis-like mice dorsal skins (A), and SDLN (D). Scale bar = 250 nm. Lambda scan was shown as spectrum in one representative experiment of skin sections (B), and SDLN sections (E). FRET ratios were calculated by the emission intensities of donor (505 nm) and acceptor (565 nm) based on formula FRET ratio = I_{565 nm}/(I_{505 nm} + I_{565 nm}) and shown data pooled from separate experiments of skin sections (C), and SDLN sections (F). Data are presented as mean ± SD (n = 9), ∗∗∗∗P < 0.0001.