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. Author manuscript; available in PMC: 2021 May 8.
Published in final edited form as: Cell Rep. 2020 Dec 1;33(9):108465. doi: 10.1016/j.celrep.2020.108465

Figure 1. Simple In Vitro System to Derive Human Hemogenic Endothelial Cells from Human Embryonic Stem Cells (hESCs).

Figure 1.

(A) Schematic representation of differentiation protocol, partially adapted from Sriram et al. (2015).

(B) qPCR analysis of markers associated with pluripotency (SOX2), primitive streak/early mesoderm (TBXT), mesoderm (HAND1), endothelium (CDH5), arterial endothelium (EFNB2), venous endothelium (EPHB4), and hematopoiesis (RUNX1); n = 3 for all groups. Student’s t test: p < 0.0001 (SOX2), p = 0.0008 (TBXT), p = 0.0253 (HAND1), p = 0.0028 (CDH5), p = 0.0018 (EFNB2), p = 0.0049 (EPHB4), p = 0.0029 (RUNX1).

(C) Flow cytometry result showed generation of endothelial cells (CD31+CD45−) and blood cells (CD45+) on day 8 (D8).

(D) Giemsa staining revealed generation of all blood cell lineages. Original magnification, 40×. Scale bar, 25 μm.

(E) Flow cytometry result showed generation of multi-lineage blood cells.

Data are represented as means ± SDs.