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. Author manuscript; available in PMC: 2021 May 8.
Published in final edited form as: Cell Rep. 2020 Dec 1;33(9):108465. doi: 10.1016/j.celrep.2020.108465

Figure 6. Bulk RNA Sequencing Reveals Candidate Mediators of RA Regulation of Human Endothelial Cell Cycle Control and Hemogenic Specification.

Figure 6.

(A) Schematic representation of experimental design.

(B) Endothelial cells (CD31+CD45−) treated with (i) n/a or (ii) RA were collected on D8 for RNA sequencing using FACS sorting.

(C) Gene set enrichment analysis was performed for genes exclusively downregulated in RA-treated endothelial cells. Relevant and significant (p < 0.05) terms are shown.

(D) Gene set enrichment analysis was performed for genes exclusively upregulated in RA-treated endothelial cells. Relevant and significant (p < 0.05) terms are shown.

(E) Selected markers demonstrate upregulation of genes associated with hematopoietic cell fate in endothelial cells by RA.