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. Author manuscript; available in PMC: 2021 May 8.
Published in final edited form as: Cell Rep. 2020 Dec 1;33(9):108465. doi: 10.1016/j.celrep.2020.108465

Figure 7. Bulk RNA Sequencing Provides Insights That Link Endothelial Cell Cycle State to Hemogenic Specification.

Figure 7.

(A) Schematic representation of experimental design.

(B) Endothelial cells (CD31+CD45−) in different cell cycle phases (early G1, late G1, G1/S, S/G2/M) were collected on D8 for RNA sequencing using FACS sorting. (C) Gene set enrichment analysis was performed for genes exclusively upregulated in early and late G1 cell cycle phases. Relevant and significant (p < 0.05) terms are shown.

(D) Gene marker expression in various cell cycle phases.

(E) Selected markers demonstrate enrichment of genes associated with hematopoiesis in early G1 phase.

(F) Selected markers demonstrate enrichment of genes associated with vascular development and arterial endothelium in late G1 phase.

(G) Schematic representation of working model.

Data are represented as the highest/lowest value within 1.5x interquartile range (IQR) above/below the 75th/25th percentile.