| Antibodies |
| Pacific Blue anti-human CD31 Antibody |
BioLegend |
Cat#303113; RRID:AB_1877196 |
| FITC anti-human CD31 Antibody |
BioLegend |
Cat#303104; RRID:AB_314330 |
| Alexa Fluor® 700 anti-human CD45 Antibody |
BioLegend |
Cat#304024; RRID:AB_493761 |
| APC/Cyanine7 anti-human CD45 Antibody |
BioLegend |
Cat#304014; RRID:AB_314402 |
| PE/Cy7 anti-human CD309 (VEGFR2) Antibody |
BioLegend |
Cat#359911; RRID:AB_2563551 |
| APC anti-human CD117 (c-kit) Antibody |
BioLegend |
Cat#313206; RRID:AB_314985 |
| PE anti-human CD144 (VE-Cadherin) Antibody |
BioLegend |
Cat#348506; RRID:AB_2077978 |
| Pacific Blue anti-human CD34 Antibody |
BioLegend |
Cat#343512; RRID:AB_1877197 |
| FITC anti-human CD20 Antibody |
BioLegend |
Cat#302303; RRID:AB_314251 |
| Brilliant Violet 421 anti-human CD3 Antibody |
BioLegend |
Cat#317343; RRID:AB_2565848 |
| Brilliant Violet 421 anti-human CD14 Antibody |
BioLegend |
Cat#325627; RRID:AB_2561342 |
| Alexa Fluor® 594 anti-human CD66b Antibody |
BioLegend |
Cat#392907 |
| Alexa Fluor® 488 anti-human CD41 Antibody |
BioLegend |
Cat#303723; RRID:AB_2566302 |
| APC anti-human CD235ab Antibody |
BioLegend |
Cat#306607; RRID:AB_314625 |
| PE anti-human CD11b Antibody |
BioLegend |
Cat#301305; RRID:AB_314157 |
| Alexa Fluor® 700 anti-human CD45 Antibody |
BioLegend |
Cat#304024; RRID:AB_493761 |
| Zombie NIR Fixable Viability Kit |
BioLegend |
Cat#423105 |
| Chemicals, Peptides, and Recombinant Proteins |
| Stemolecule CHIR99021 |
Stemgent |
Cat#04–0004 |
| Recombinant Human FGF basic/FGF2/ bFGF (146 aa) Protein |
R&D systems |
Cat#233-FB-025 |
| Recombinant Human BMP-4 Protein |
R&D systems |
Cat#314-BP-010 |
| Recombinant Human VEGF165 |
PeproTech |
Cat#100–20 |
| Retinoic acid |
Sigma-Aldrich |
Cat#R2625–50MG |
| AGN 194310 |
MedChemExpress |
Cat#HY-16681 |
| PD 0332991 isethionate |
Sigma-Aldrich |
Cat#PZ0199–5MG |
| CVT-313 |
Santa Cruz Biotechnology |
Cat#199986–75-9 |
| Giemsa stain, modified |
Sigma-Aldrich |
Cat#GS500–500ml |
| Normocin - Antimicrobial Reagent |
InvivoGen |
Cat#ant-nr-1 |
| Fibronectin Human Protein, Plasma |
GIBCO |
Cat#33016015 |
| Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free, 5 mL |
Corning |
Cat#356230 |
| Critical Commercial Assays |
| RNeasy Plus Micro Kit |
QIAGEN |
Cat#74034 |
| 5X All-In-One RT MasterMix |
abm |
Cat#G490 |
| miScript SYBR® Green PCR Kit |
QIAGEN |
Cat#218073 |
| Dil-Ac-LDL Kit |
Cell Applications |
Cat#022K |
| Deposited Data |
|
|
| RNA-seq-1 |
This paper |
GSE159694 |
| RNA-seq-2 |
This paper |
GSE159694 |
| Experimental Models: Cell Lines |
|
|
| H1-hESC |
Yale Stem Cell Center |
N/A |
| H9-Fucci-hESC |
Pauklin and Vallier, 2013 |
N/A |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: B6.Cg-Tg(FucciG1)#596Bsi |
Riken |
Cat#RBRC02707 |
| Mouse: C57BL/6J |
JAX |
Cat#000664 |
| Oligonucleotides |
|
|
| See Table S1 for oligonucleotide sequences |
N/A |
N/A |
| Software and Algorithms |
|
|
| Prism 7 |
Graphpad Software |
https://www.graphpad.com/scientific-software/prism/ |
| ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
| bcl2fastq2_v2.19.0 |
Illumina |
https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software.html |
| Galaxy |
Afgan et al., 2018 |
https://usegalaxy.org/ |
| DESeq2 |
Love et al., 2014 |
N/A |
| ComplexHeatmap |
Phipson et al., 2016 |
N/A |
| limma |
Phipson et al., 2016 |
N/A |
