Skip to main content
. 2021 Apr;9(8):631. doi: 10.21037/atm-20-7084

Figure 4.

Figure 4

Pretreatment with 3-MA exacerbates the IR-induced acute liver injury in mice pretreated with lycopene. (A) Serum ALT and AST in mice with IR injury in different groups (n=6 mice/group). (B) Representative hematoxylin and eosin (H&E) staining of liver tissue sections for different groups (scale bars, 250 µm). (C,D) The average Suzuki scores and liver myeloperoxidase (MPO) activities for different groups. (E,F) TUNEL staining of liver sections (scale bars, 200 µm) and the relative ratios of TUNEL-positive cells for different groups. (G) Relative caspase-3 activity was evaluated from total lysates of IR lobes. (H) Western blot analysis of the protein levels of Bcl-2, Bcl-xL, and β-actin in different groups. All data represent the mean ± SD, *P<0.05. IR, ischemia reperfusion; ALT, alanine aminotransferase; AST, aspartate aminotransferases.