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. 2021 Apr;9(8):712. doi: 10.21037/atm-21-1448

Figure 6.

Figure 6

Knockout of Nrf2 gene eliminated the protective effect of 4-OI on MRSA-induced ALI. (A) HE staining (magnification, ×200, bar =50 µm; n=5). (B) Mouse lung injury score. (C) Total number of cells in BALF. (D) Protein contents in BALF (n=5). (E) Activity of MPO in lungs (n = 3). (F) MPO immunohistochemistry (magnification, ×200, bar =50 µm; n=5). (G) Semiquantitative analysis of MPO content by immunohistochemistry. (H) Determination of MRSA bacterial burden in lung tissue. (I) Effect of 4-OI on survival curves of MRSA-induced sepsis in Nrf2−/− mice (n=7–10/group). Data represent the mean ± standard deviation (*, P<0.05; ***, P<0.001). NS, no significant difference. MRSA, methicillin-resistant Staphylococcus aureus; 4-OI, 4-octyl itaconate; ALI, acute lung injury; MPO, myeloperoxidase; BALF, bronchoalveolar lavage fluid.