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. 2021 Apr;9(8):627. doi: 10.21037/atm-20-6891

Figure 4.

Figure 4

Effects of apelin-13 on LC3B expressions in VSMCs of WKY and SHR. LC3B expression was determined with immunofluorescence staining. The measurements were carried out 24 h after PBS, apelin-13 (1 µM) or F13A (1 µM) treatment. (A) Relative fluorescence intensity of LC3B normalized with cell area. (B) Representative images of triple immunofluorescence staining for LC3B (red), α-SMA (green) and DAPI (blue). The α-SMA and DAPI staining were respectively used to show VSMC cells and nuclei. Values are mean ± SE. *, P<0.05 vs. WKY; †, P<0.05 vs. PBS; ‡, P<0.05 vs. Apelin-13. n=5. α-SMA, α-smooth muscle actin; DAPI, 4',6-diamidino-2-phenylindole.