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. 2021 Apr;9(8):687. doi: 10.21037/atm-21-980

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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CD2⁺ cells differentiate from CD133⁺ cells and exhibit stronger stem-like properties. (A) The process of CD133⁺ cells differentiation into CD2⁺ cells observed by flow cytometry. The representative result is shown. (B,C) Sphere formation of different types of A549 and Lewis cells, with the sphere-forming capability evaluated based on the spheroid diameter and the number of spheroids. Scale bar: 100 µm. n=5 wells per group. Real-time PCR (D) and western blot (E) detection of pluripotency factors in 3 types of cells. Gene expression was normalized to the geometric mean of the housekeeping gene GAPDH. Data are shown as mean ± SD. All experiments were repeated at least 3 times. **, P<0.01; ***, P<0.001. Sox2, SRY-related high-mobility-group (HMG)-box protein-2; Oct4, octamer-binding transcription factor-4; PCR, polymerase chain reaction.