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. 2021 Apr;9(8):659. doi: 10.21037/atm-21-935

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Upregulation of CK1 was associated with poor prognosis and was involved in glioma cell behavior. (A) Representative images of CK1 staining by immunohistochemistry in tumor tissues from patients diagnosed with grade II and grade IV glioma (200×). The red arrow indicates a significant difference (P<0.05). (B) Kaplan-Meier survival curve for glioma patients stratified by low and high CK1 expression. (C) Representative western blots showing the expression levels of CK1 in U251, U373, and U87MG glioma cell lines. The gray value statistics graph is shown below. * indicates a significant difference (P<0.05). (D) Cell viability of glioma cell lines analyzed at 24, 36, 48, and 60 hours. (E) The wound closure ability of cell lines was analyzed in scratch experiments (200×).