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. 2021 Apr;9(8):675. doi: 10.21037/atm-21-997

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Circ0097009 functions as a sponge for microRNA-1261 (miR-1261). (A) Predicted direct binding sites of miR-1261 within the circ0097009 sequence. (B) Expression level of miR-1261 in the LO2 cell line and hepatocellular carcinoma cell lines. (C) Relative luciferase activity of HepG2 cells co-transfected with the miR-1261 mimics and the luciferase reporter vector containing the wild-type or mutant circ0097009 sequence. (D) MS2-based RNA immunoprecipitation assay in HepG2 cells transfected with MS2bs-circ0097009, MS2bs-circ0097009-mt, or MS2bs-Rluc. **P<0.01.