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. 2021 Apr;9(8):675. doi: 10.21037/atm-21-997

Figure 5.

Figure 5

Knockdown of circ0097009 promotes ferroptosis in hepatocellular carcinoma (HCC) cells. (A) Predicted direct binding sites of microRNA-1261 (miR-1261) within the solute carrier family 7 member 11 (SLC7A11) sequence. (B) Expression level of SLC7A11 in the LO2 cell line and HCC cell lines. (C) Relative luciferase activity of HepG2 cells co-transfected with miR-1261 mimics and the luciferase reporter vector containing the wild-type or mutant SLC7A11 sequence. (D) Expression level of SLC7A11 after transfection with miR-1261 mimics. (E) Western blotting was performed to determine the expression levels of SLC7A11, glutathione peroxidase 4, and GAPDH. (F) RNA immunoprecipitation (RIP) assay detected the relative enrichment of circ0097009, SLC7A11, and miR-1261 in the anti-Argonaute 2 (Ago2) fraction (left); relative enrichment was detected by an Ago2-RIP assay (right). (G) Expression status of SLC7A11 in hematoxylin-eosin-stained sections of harvested xenograft tumors. (H) Glutathione (GSH)/oxidized GSH (GSSG) ratio was determined with a GSH/GSSG Quantification Kit. **P<0.01.