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. 2021 Apr;9(8):671. doi: 10.21037/atm-21-597

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Neural stem cell (NSC) culture, identification, and differentiation in vitro. (A) Change in cell morphology within 7 days during the differentiation process. Bar =50 µm. (B) Expression of miR-140-5p after NSC differentiation. (C) Identification of NSCs, neural precursor cells, astrocytes, and neurons after differentiation, using nestin (green), glial fibrillary acidic protein (GFAP) (green), doublecortin (DCX) (green), and MAP-2 (red) markers. Bar =50 µm. Data are presented as the mean ± SEM (standard error of mean) (*P<0.05).