Skip to main content
. 2021 Apr;9(8):671. doi: 10.21037/atm-21-597

Figure 4.

Figure 4

Overexpression of miR-140-5p affected the proliferation rate, not the ratio of neural stem cells (NSCs). (A) 4,6-diamino-2-phenyl indole (DAPI) (blue) + 5-Bromo-2-deoxyUridine (BrDU) (red) immunofluorescence staining in the five groups. (B) DAPI (blue) + nestin (green) + ki-67 (red) immunofluorescence staining in the five groups. (C) CCK-8 result in the five groups. (D) Method used to measure the diameter of neural spheres. (E) Comparison of neural sphere diameters 12, 24, and 48 h after transfection in the five groups. Data are presented as the mean ± SEM (*P<0.05). Bar =50 µm.