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. 2021 Apr;9(8):671. doi: 10.21037/atm-21-597

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Overexpression of miR-140-5p inhibited differentiation into neurons and promoted the differentiation of neural stem cells (NSCs) into astrocytes. (A) 4,6-diamino-2-phenyl indole (DAPI) (blue) and doublecortin (DCX) (green) staining of NSCs after 7 days of differentiation in the five groups. (B) DAPI (blue) and map-2 (red) immunofluorescence of NSCs after differentiation. (C) DAPI (blue) and glial fibrillary acidic protein (GFAP) (green) result after 7 days of differentiation in the five groups. Bar =50 µm. Data are presented as the mean ± SEM (*P<0.05).