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. 2021 Apr;9(8):671. doi: 10.21037/atm-21-597

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Overexpression of miR-140-5p inhibited the downstream target of transcription factor Prox1. (A) Immunofluorescence result of 4,6-diamino-2-phenyl indole (DAPI) (blue) and Prox1 (red) in the five groups. The arrow markers represent Prox1+ cells. (B) The 3' non-coding region of PROX1 mRNA is completely complementary to the 6-nucleotide seed sequence at the 5' end of miR-140-5p, which is the predicted target site region. (C) Western blot analysis result of Prox1 in the five groups. Data are presented as the mean ± SEM (*P<0.05). Bar =50 µm.