Fig. 1.

Phylogenetic analysis and CDR3 alignment of anti–Etf-1 Nbs. (A) Phylogenetic analysis of 24 distinct anti–Etf-1 Nbs based on their CDR3 amino acid sequences. Redundancy and family classification are shown on the right. Font colors indicate Nbs that bind denatured Etf-1 (black), nondenatured Etf-1 (green), or both (magenta). (B) Amino acid sequence alignment of anti–Etf-1 Nbs. CDR3 amino acid sequences are highlighted in yellow, and the number of amino acids in each CDR3 is shown on the right.