Fig. 7.
D7, but not D3, abrogates the increase in MnSOD and attenuation of intracellular ROS generation by Etf-1. HEK293 cells were transfected with Etf-1–HA, or cotransfected with Etf-1–HA and D7 or D3. Control, untransfected cells. (A) At 2 dpt, cell lysates were subjected to Western blotting using antibodies against HA, MnSOD, and human actin. (B and C) Quantification of relative band densities of MnSOD (B) and Etf-1 (C) normalized against actin. (D) ROS production at 2 dpt was analyzed by the fluorescent indicator H2DCFDA. Null, buffer control without H2DCFDA. (B−D) Data are presented as the mean ± SD from three independent experiments with triplicates per sample. *P < 0.05, by one-way ANOVA.