Table 1.

Summary of mutations in coding regions found in the isogenic transplant litterbags

Gene Name	Position	Specific mutation	Type of mutation	Occurrences in samples†	Mutation frequency‡	Gene description
relA →	732,949	G → T	G196V	All samples	62.0 ± 20.0%	Bifunctional (p)ppGpp
		nonsynonymous	(GGC → GTC)			synthase/hydrolase
marR →	1,513,025	A → C	A102A	Scrubland T1;	32.1 ± 7.0%	Multiple antibiotic
		synonymous	(GCA → GCC)	Pine–Oak T1-T3;		resistance protein
				Subalpine T1-T3
orf01789 ←	1,895,438	Δ54 bp	—	Desert T1-T3;	12.8 ± 1.8%	Zinc transporter
		deletion		Scrubland T1,T3;
				Grassland T1-T3;
				Pine–Oak T1,T3;
				Subalpine T1,T3
lutR →	2,623,478	G → A	G77E	Subalpine T2-T3	13.4 ± 0.7%	HTH-type transcriptional
		nonsynonymous	(GGG → GAG)			regulator
wcaJ →	3,114,825	C → T	Q182*	Desert T1-T3;	30.6 ± 6.2%	DP-glucose:undecaprenyl-
		nonsense	(CAG → TAG)	Scrubland T2-T3;		phosphate glucose-1-
				Grassland T1-T3		phosphate transferase
lacF ←	3,355,556	Δ46 bp	—	all samples	14.7 ± 2.8%	Lactose transport system
		deletion				permease protein

^†T1 = population sample collected at 6 mo, T2 = 12 mo, and T3 = 18 mo.

^‡Frequency calculated from samples where mutation was detected.