Cortical surface projection mapping delineates cortical areas. Cortical area marker genes were projected onto the cortical surface and pseudocolored to compare the expression patterns of multiple genes. (A and B) Cortical surface projection mapping of ANOS1. (C and D) Cortical surface projection mapping of UNC5D. (E and F) Overlay image of ANOS1 and UNC5D expression. (G and H) Cortical surface projection mapping of WHRN. (I and J) Overlay image of ANOS1 and WHRN expression. (K and L) Overlay image of ANOS1, WHRN, and UNC5D expression. (M–O) Lateral views of WIF and DCN expression (M), CUX1 and DCN expression (N), and WIF, CUX1, and DCN expression (O). Cortical surface projection mapping delineates the A1/2, A3a/b, A4b, A6, A8, PE/PG (parietal area), DM (dorsomedial visual area), V1, V2, V3, V4, MT (V5), AuA1 (primary auditory cortex), A36/TE1/STR (temporal area 1, superior temporal area), TE2/TE3, MST (medial superior temporal area), and V4T (visual area 4, transitional part) areas.