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. Author manuscript; available in PMC: 2021 May 8.
Published in final edited form as: Cell Mol Bioeng. 2012 Feb 4;5(2):184–193. doi: 10.1007/s12195-012-0220-4

Figure 4. Sphingosine-1-phosphate reduces permeability but S1P receptor inhibition does not alter the MoDC-driven reduction in permeability.

Figure 4.

(A) Pre-treatment with S1P for 1 h produced a dose-dependent reduction in endothelial monolayer permeability to albumin that was abrogated by the S1P-1 and -3 receptor inhibitor VPC-23019 (1 μM) (n=4 independent experiments). (B) Non-contact co-culture of monolayers with MoDC for 5 days significantly reduced endothelial permeability (P=0.0254; n=3 independent experiments). Pre-treatment with VPC-23019 (1 μM) for 1 h before the addition of tracer did not significantly alter permeability in the presence or absence of MoDC (P=0.246). *P <0.05, **P<0.01, ***P<0.001.