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. 2021 Mar 5;49(2):563–578. doi: 10.1042/BST20200163

Figure 5. SBPs for abundant plant-derived oligosaccharides by B. animalis subsp. lactis Bl-04.

Figure 5.

(A) The overall structure of the arabinoxylooligosaccharide binding protein BlAXBP in complex with xylotetraose (PDB: 3ZKK). (B) and (C) Recognition of arabinoxylotriose (PDB: 4C1T) and arabinoxylobiose (PDB: 4CIU), respectively, with the amino acid residues coloured according to the domain colour as in (A). The arabinosyl sidechains (orange) can be accommodated in cavities in the binding protein, and the structure view in (C) is tilted approximately 90° for clarity. (D) The overall structure of the SBP BlG16BP that mediates the capture of both α-1,6-linked galactosides and glucosides in bound form with the preferred ligand panose (PDB: 4ZZE). (E,F) Details of binding panose and raffinose (PDB: 4ZS9), respectively. The recognition motif of BlG16BP is the disaccharide unit at positions 1 and 2, and the open binding site allows the accommodation of different monosaccharide units at position 3 (Glc and Fru at position 3 are shown in orange).