Table 2.

Checkerboard analysis of zymosan-induced MC migratory response

Zymosan in the bottom well (μg/ml)	Zymosan in top well (μg/ml)
	0	1	10	50
0	100 ± 6	176 ± 25	506 ± 44	682 ± 21
1	139 ± 10**	435 ± 33	646 ± 42	366 ± 13
10	376 ± 14***	368 ± 13	330 ± 18	295 ± 27
50	286 ± 12***	292 ± 24	513 ± 25	410 ± 36

MCs were stimulated with various concentrations of zymosan or medium alone (control spontaneous MC migration) for 3 h at 37°C in a Boyden microchamber. Ten HPF were counted in each assay (×250). Spontaneous cell migration served as a control and was identified to be 100%. Results are shown as the mean ± SD of three independent experiments, and each experiment was carried out in duplicate samples (each experiment was performed on 2 animals/MC isolates). **P < 0.01, ***P < 0.001