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. Author manuscript; available in PMC: 2022 May 21.
Published in final edited form as: Neuroscience. 2021 Feb 2;463:227–237. doi: 10.1016/j.neuroscience.2021.01.025

Figure 3.

Figure 3.

Validation of PVN transfection and EGFR knockdown. A lentiviral vector encoding a scrambled (Scr) siRNA + green fluorescent protein (GFP) or EGFR siRNA + GFP was microinjected bilaterally in PVN. One week later, GFP was distributed diffusely throughout the PVN (A). A normal control rat is shown for comparison. Rats treated with the EGFR siRNA had significantly lower levels of EGFR mRNA, compared with normal control rats and rats treated with the Scr siRNA (B). Values in (B) are mean ± SEM (n = 6 for each group) and are expressed as a fold change compared to Control. *p<0.05, vs Control or Scr siRNA.