Table 1.
Gene | Primers | Sequences |
---|---|---|
BIP | Forward primer: Reverse primer: |
5’-AAGGTGAACGACCCCTAACAAA-3’ 5’-GTCACTCGGAGAATACCATTAACATCT-3’ |
ATF4 | Forward primer: Reverse primer: |
5’-CTACTAGGTACCGCCAGAAG-3’ 5’-GCCTTACGGACCTCTTCTAT-3’ |
ATF6 | Forward primer: Reverse primer: |
5’-GATTTGATGCCTTGGGAGTC-3’ 5’-GGACCGAGGAGAAGAGACAG-3’ |
TNF-α | Forward primer: Reverse primer: |
5’-CCTTATCTACTCCCAGGTTCTC-3’ 5’-TTTCTCCTGGTATGAATGGC-3’ |
IL-1β | Forward primer: Reverse primer: |
5’-CGACAGAATCTAGTTGTCC-3’ 5’-TCATAAACACTCTCATCCACAC-3’ |
COX-2 | Forward primer: Reverse primer: |
5’-GGCACAAATATGATGTTCGCA-3’ 5’-CCTCGCTTCTGATCTGTCTTGA-3’ |
ACE | Forward primer: Reverse primer: |
5’-GTGTTGTGGAACGAATACGC-3’ 5’-CCTTCTTTATGATCCGCTTGA-3’ |
AT1aR | Forward primer: Reverse primer: |
5’-ACTCACAGCAACCCTCCAAG-3’ 5'-ATCACCACCAAGCTGTTTCC-3' |
β-actin | Forward primer: Reverse primer: |
5’-CCGCGAGTACAACCTTCT-3’ 5'-CGTCATCCATGGCGAACT-3' |
BIP: binding immunoglobulin protein; ATF4: activating transcription factor 4; ATF6: activating transcription factor 6; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β; COX-2: cyclooxygenase-2; ACE: angiotensin converting enzyme; AT1aR: angiotensin II type 1a receptor.