Skip to main content
. Author manuscript; available in PMC: 2022 May 21.
Published in final edited form as: Neuroscience. 2021 Feb 2;463:227–237. doi: 10.1016/j.neuroscience.2021.01.025

Table 1.

Sequences for primers

Gene Primers Sequences
BIP Forward primer:
Reverse primer:
5’-AAGGTGAACGACCCCTAACAAA-3’
5’-GTCACTCGGAGAATACCATTAACATCT-3’
ATF4 Forward primer:
Reverse primer:
5’-CTACTAGGTACCGCCAGAAG-3’
5’-GCCTTACGGACCTCTTCTAT-3’
ATF6 Forward primer:
Reverse primer:
5’-GATTTGATGCCTTGGGAGTC-3’
5’-GGACCGAGGAGAAGAGACAG-3’
TNF-α Forward primer:
Reverse primer:
5’-CCTTATCTACTCCCAGGTTCTC-3’
5’-TTTCTCCTGGTATGAATGGC-3’
IL-1β Forward primer:
Reverse primer:
5’-CGACAGAATCTAGTTGTCC-3’
5’-TCATAAACACTCTCATCCACAC-3’
COX-2 Forward primer:
Reverse primer:
5’-GGCACAAATATGATGTTCGCA-3’
5’-CCTCGCTTCTGATCTGTCTTGA-3’
ACE Forward primer:
Reverse primer:
5’-GTGTTGTGGAACGAATACGC-3’
5’-CCTTCTTTATGATCCGCTTGA-3’
AT1aR Forward primer:
Reverse primer:
5’-ACTCACAGCAACCCTCCAAG-3’
5'-ATCACCACCAAGCTGTTTCC-3'
β-actin Forward primer:
Reverse primer:
5’-CCGCGAGTACAACCTTCT-3’
5'-CGTCATCCATGGCGAACT-3'

BIP: binding immunoglobulin protein; ATF4: activating transcription factor 4; ATF6: activating transcription factor 6; TNF-α: tumor necrosis factor-α; IL-1β: interleukin-1β; COX-2: cyclooxygenase-2; ACE: angiotensin converting enzyme; AT1aR: angiotensin II type 1a receptor.