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. Author manuscript; available in PMC: 2022 May 6.
Published in final edited form as: Cell Stem Cell. 2021 Feb 24;28(5):894–905.e7. doi: 10.1016/j.stem.2021.02.003

Figure 3. H3.3G34R and co-occurring mutations alter gene expression profile.

Figure 3.

(A) Principal component analysis of RNA-seq data shows a distinct expression profile of DKO-G34R cells, which are clustered together with H3.3G34R-mutant patient-derived cell lines (SJHGGx6 and HSJD002) (upper left). Hindbrain cells form a separate cluster regardless of mutation status (upper right).

(B) RT-qPCR confirms the altered expression of the indicated genes by the combination of mutations in vFNPCs, but not in vHNPCs (n = 3–5).

P values were calculated by two-sided Welch’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.